magnesium-dependent protein serine/threonine phosphatase activity, protein serine/threonine phosphatase activity 6a) were incubated with 0.5 µl λ-protein phosphatase … PPP1R2C is a heat-stable protein that acts as an inhibitor for protein phosphatase-1 (PP1; see 176875) (Shirato et al., 2000).Although PPP1R2C had been considered a nonfunctional pseudogene designated PPP1R2P9, later evidence suggested that it encodes a functional protein (Korrodi-Gregorio et al., 2013). Protein phosphatase 2C (PP2C) is a member of the metal-dependent protein phosphatases (PPMs). Here, we show that the Arabidopsis thaliana protein phosphatase type 2C, ABI1, a negative regulator of abscisic acid signaling, is involved in the regulation of ethylene biosynthesis under oxidative stress conditions. During abscisic acid (ABA) signaling, these inhibitor proteins comprise PYR1/PYL/RCAR ABA receptors, which inhibit the core negative regulators, the clade A type 2C protein phosphatases (PP2Cs). Here, we report the identification and functional characterization of a novel type 2C protein phosphatase, CaADIP1 (Capsicum annuum A BA and D rought‐ I nduced P rotein phosphatase 1 Few targeting proteins have yet been identified and, to date, there are no PP2C inhibitors available.
Protein phosphatases are usually negatively regulated by inhibitor proteins. An abscisic acid (ABA)-induced cDNA fragment encoding a putative protein phosphatase 2C (PP2C) was obtained by means of differential reverse transcriptase-polymerase chain reaction approach.

For protein dephosphorylation, 20 µl of a peroxisomal fraction from a ptc5 mutant expressing Gpd1-GFP (see Supplementary Fig. The diagram represents the Arabidopsis ABI1, ABI2, AtP2C-HA, AthPP2CA, and KAPP proteins, as well as the alfalfa MP2C protein.

PDC, pyruvate dehydrogenase complex, PP2C, protein phosphatase type‐2C, RT‐PCR, reverse transcriptase‐polymerase chain reaction, ZMPP2, Zea mays protein phosphatase 2C. Protein phosphatase 2C (PP2C) dephosphorylates a broad range of substrates and regulates apoptosis, stress response and growth-related pathways. PP2C is a monomeric enzyme with at least four isoforms in human. In the course of screening for PP2C activators from natural sources, we isolated abietane-type diterpenes, pisiferdiol and pisiferic acid from Chamaecyparis pisifera . Abscisic acid (ABA) mediates resistance to abiotic stress and controls developmental processes in plants. Abscisic acid (ABA) is a key phytohormone that regulates plant growth and developmental processes, including seed germination and stomatal closing. Schematic diagram of plant type-2C protein phosphatases. The full-length clone was isolated from a cDNA library constructed using mRNA from ABA-treated beechnut ( Fagus sylvatica ) seeds. As a result, it has been difficult to examine the role of this phosphatase. EAR1 Negatively Regulates ABA Signaling by Enhancing 2C Protein Phosphatase Activity[OPEN] Kai Wang,a,1 JunnaHe,a,b,1 Yang Zhao,c,d TingWu,a Xiaofeng Zhou,a,b Yanglin Ding,a LingyaoKong,a Xiaoji Wang,a YuWang,a Jigang Li,a Chun-Peng Song,e Baoshan Wang,f Shuhua Yang,a Jian-Kang Zhu,c,d and Zhizhong Gonga,2 a State Key Laboratory of Plant Physiology and Biochemistry, College …

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